Statistical modeling and simulation for differential expression analysis of sequenced databases

The development of single-cell RNA-sequencing (scRNA-seq) has made it feasible to quantify the dynamics of gene expression at the single-cell level. A significant amount of expression data for thousands or even millions of genes across millions of cells can be produced in a single experiment using scRNA-seq. The main downstream analysis of this type of data to find gene markers for cell type detection and to supply inputs for other secondary investigations is differential expression analysis....

New strategies for chemical solution deposition of YBa2Cu3O7-δ film towards fusion applications

The applications of superconducting materials are very wide-ranging and concern energy technologies, medical and analytical instruments, fundamental and applied research. In the field of nuclear fusion, superconductors are defined as enabling technology because the creation of a tokamak fusion machine for application purposes such as commercial power plant would not be possible without the use of superconducting materials to effectively confine the hot plasma generated in the reactor. The dev...

Development of FRDA iPSCs-derived Cardiomyocytes as a tool to evaluate efficacy of potential therapeutic compounds

Friedreich ataxia is a disease mainly due to the low expression of frataxin protein with consequent production of ROS and free iron accumulation. Since one of the major causes of death is associated with cardiac hypertrophy, it is of crucial importance to develop a cellular cardiac model that specifically recapitulates cardiac phatophysiology of FA. Therefore, our aim concerns the generation of a robust method of differentiation and characterization of cardiomyocytes derived from patient iPSC...

Il decreto di litis contestatio: i poteri del giudice sulla formula del dubbio

Oggetto del presente lavoro è lo studio del decreto di litis contestatio nel processo matrimoniale canonico e dei poteri del giudice in ordine alla sua determinazione e alla sua modificazione. Come noto, il decreto di litis contestatio è emesso dal giudice al termine della fase introduttiva di ogni giudizio canonico e ha come scopo precipuo quello di determinare la fissazione dei termini della controversia, in tal modo costituendo l’ambito e provvedendo al tempo stesso ai limiti del successiv...

Development of multi-responsive phase change contrast agents with hybrid shells of potential theranostic relevance

Recently, in medicine, there has been much focus on the early screening of diseases such as tumours. Screening refers to the use of simple tests across an apparently healthy population in order to identify individuals who have risk factors or early stages of disease, but do not yet have symptoms. This technique has some limitations [1]: - It can involve cost and use of medical resources on a majority of people who do not need treatment; - Adverse effects of screening procedure (e.g. stress...

Multi-sensor epidermal electronics for vital signs monitoring: application to core body temperature and respiratory function

Monitoring of key vital signs is crucial to provide a valuable insight into a person’s health, to make clinical deterioration preventable by a timely intervention. However, it makes use of sophisticated equipment, uncomfortable for the patients, and mainly limited to in-hospital use. Wearable devices, that are intended to be worn and carried around with limited restraints, can overcome these issues, although still with some limitations in terms of lightweight and power requirement. Accordingl...

Limit analysis of dry-masonry blocks structures: experimental and numerical investigation

Within the theoretical framework of limit analysis, this thesis examines some problems arising in the assessment of the collapse mechanisms of historical masonry structures, featured by dry mortar joints. The attention is focused on the constitutive laws that govern the frictional contact behaviour, taking into account both associative and nonassociative flow laws. Under the classical Heyman assumptions, the response of vaulted systems on spreading supports is investigated. Applying the lower...

Comparison of two different screws for rapid maxillary expansion. A randomized multicentric study

Identification of genomics signatures of drug response and tumorigenesis

Recent advances in next-generation sequencing (NGS) have generated a wealth of data of different types whose analysis have helped in the identification of signatures of different cellular sensitivity/resistance responses to hundreds of chemical compounds. Among the different data types, gene expression has proven to be the most successful one for the inference of drug response in cancer cell lines. Although effective, the whole transcriptome can introduce noise in the predictive models, since...