In questo lavoro tecniche di monitoraggio tradizionale e molecolare, attraverso metabarcoding di DNA ambientale, sono state cooptate per l'analisi della biodiversità zoologica del bacino fluviale del Serchio. Dati già presenti in letteratura sono stati complementati con waypoints ottenuti da monitoraggio tradizionale effettuato su specie di interesse erpetologico. DNA ambientale acquatico è stato prelevato da vari punti strategici nel bacino, ed i risultati confrontati con quelli ottenuti att...
Breast cancer (BC) is a heterogeneous disease with distinct molecular subtypes differing in biological behavior and clinical outcome. A major driver of this heterogeneity is the presence of cancer stem cells (CSCs), endowed with self-renewal and tumor-initiating capacity, contributing to progression, relapse, and therapy resistance. This project investigated the link between stemness, tumor heterogeneity, and metabolic plasticity in BC using cell lines and patient-derived cultures from primar...
Major depressive and bipolar disorders are prevalent mental health conditions sharing the presence of major depressive episodes (MDEs). While psychopharmacological and psychological therapies are first-line treatments for MDEs, the response is often incomplete. New approaches focused on the human-nature relationship might complement antidepressant treatments, improving response. This PhD thesis aims to assess the feasibility of implementing regular exposure to green environments such as woods...
Multiple sclerosis (MS) is a disease of the central nervous system characterized by chronic inflammation and immune-driven demyelination. To date, the etiopathogenesis of the disease remains unclear, although it is believed that a combination of genetic and environmental factors contributes to its onset. Among the environmental factors, growing evidence highlights the role of the gut microbiota in both the onset and progression of the disease. Indeed, the gut microbiota constitutes a complex ...
The conservation of local and endangered livestock breeds is crucial to maintaining biodiversity and ecosystem services, while ensuring their resilience and adaptability to harsh environments. Within this framework, understanding the molecular mechanisms underlying reproduction and stress adaptation becomes essential for improving welfare and management strategies. MicroRNAs (miRNAs) are small, non-coding RNA molecules that regulate gene expression at the post-transcriptional level. When rele...
Questo studio osservazionale prospettico ha esaminato la concordanza di due metodi—un saggio cromogenico anti-Xa e la LC-MS/MS—per la quantificazione delle concentrazioni plasmatiche di anticoagulanti orali nuovi (NOACs) in un contesto reale di pronto soccorso (PS). La popolazione dello studio comprendeva 80 pazienti (44 donne, 36 uomini; età mediana 85 anni) in terapia cronica con NOAC (apixaban, dabigatran, edoxaban o rivaroxaban) che si sono presentati al PS dell'IRCCS Policlinico San Matt...
Background and Aims: Cardiogenic shock (CS) mortality remains high, ranging from 30 to 60% in-hospital and from 40 to 60% at 1 year. The aim of the present study is to determine the survival (at 30 days and 1 year) and the predictors of 30-day mortality in a cohort of patients treated with temporary mechanical circulatory support (MCS) devices for CS.
Methods: Retrospective observational study including adult patients treated with temporary MCS devices for CS in a tertiary level cardiothoraci...
Objective: To delineate, within the framework of current clinical practice and criteria, the sustainability of first-line immuno-suppressive treatment discontinuation in rheumatoid arthritis (RA) and the impact of residual disease in remission on long-term drug-free (DF) outcomes.
Methods: RA patients, referring to the Pavia Early Arthritis Clinic (EAC) between 2009 and 2021 and achieving remission after DAS-driven methotrexate (MTX) monotherapy, were recruited. Eligible patients underwent D...
L'asma grave pediatrico è una malattia cronica eterogenea caratterizzata da frequenti esacerbazioni e
limitazioni significative della qualità di vita. Nonostante i progressi terapeutici, persistono ancora
importanti unmet needs nella comprensione dei meccanismi patogenetici, nell’identificazione di
possibili biomarcatori e nella gestione ottimale di questa condizione.
Abbiamo riscontrato una prevalenza complessiva dell'asma grave nei bambini del 3% senza
differenze significative tra maschi e ...