Multilayer hydrogels as potential strategy for in situ drug and/or cell delivery

A multifunctional hydrogel acting as a reservoir system to deliver specific biomolecules to targeted ischemic sites was designed in the form of a multilayer composite hydrogel integrating conventional and advanced methods (i.e., electrospray-based technique). Protocols for scale-up of hydrogel system components were developed. Furthermore, gelatin particles were developed, loaded with a model protein (BSA) and analyzed. Gelatin particles were properly functionalized. Gelatin particles were also loaded with VEGF and PlGF and analyzed. The effects of the VEGF and PlGF released from GPs/multilayer system on the cell behavior, as well as the capillary like tube formation by HUVECs, were properly measured. The optimized system was designed taking into account the rheological/mechanical results, as well as the structural/functional features. The developed system showed adequate mechanical/rheological, functional and biological properties.