Valutazione degli effetti di low level laser thetapy su fibroblasti: studio in vitro

Low-level laser therapy (LLLT) has been reported to enhance wound healing. Activation of fibroblasts has a potential for early wound healing in oral cavity. The aim of this study was to determine adequate energy doses using specific parameters of LLLT to produce biostimulatory effects on human fibroblasts culture. Materials and Method: cells were seeded on multiwell plates and exposed to a 980 nm diode laser with energy doses of 0,5, 1, 2, 4, 5, 7, 17, 50 J/cm2. Cell metabolism was evaluate by using 3-(4, 5-dimethylithiazol-2-yl)-2, 5-diphenyl tetrazolium bromide (MTT). Cell proliferation was measured by sulforhodamine B (SRB) assay. Data were analyzed by variance test with statistical significance of 5% . Results: irradiation of the fibroblasts with 2, 4 and 5 J/cm2 resulted in significant increase in cell metabolism compared with non-irradiated group (P˂ 0,05), 2 J/cm2 was found to be an optimal energy density. Cell proliferation was significantly stimulated at 2 J/cm2 . Conclusions: LLLT stimulates cell metabolism and proliferation of fibroblasts in vitro.