ENZYMATIC AND MODERN MECHANICAL APPROACHES TO ISOLATE, AND CHARACTERIZE STROMAL VASCULAR FRACTION TO STUDY ADIPOGENESIS IN VITRO AND IN VIVO FOR ITS POSSIBLE APPLICATION IN RECONSTRUCTIVE AND REGENERATIVE MEDICINE

Element-based graph pangenome

Exploring promising biomarkers in multiple sclerosis

Multiple sclerosis (MS) is a chronic inflammatory disease of the central nervous system (CNS), affecting 2.3 million people worldwide, of which approximately 122,000 are in Italy. MS represents a multifactorial disease, typical of in young adulthood, whose cause is still unknown. An important role in the MS pathogenesis, seems to be played by inflammatory processes affecting the CNS associated with neurodegenerative phenomena with consequent alteration or loss of sensory, motor and cognitive ...

Competence-based VET curriculum enactment and its implications for vocational teachers and trainers’ practices and competence in Italy and Lithuania

Basandosi su casi di implementazione del curricolo di Istruzione e Formazione Professionale (IeFP) in Lituania e in Italia, la tesi offre una nuova prospettiva sulla contemporaneità e sulla rilevanza del curricolo basato sulle competenze (competence-based curriculum) per la pratica della IeFP e per gli studenti, sui suoi elementi definitori e sulle possibili variazioni a livello teorico-concettuale e normativo-governativo. Il modello di curricolo basato sulle competenze è applicato nei sistem...

Principio pattizio e rigidità costituzionale: dalle Chartes francesi alla realtà sovranazionale.

This PhD dissertation attempts to approach the concept of constitutional rigidity from the specific point of view of conventional sources: particular attention is devoted to the constitutionalism of the 19th Century, mainly to Statuto albertino and French Chartes of 1814 and 1830: thereafter, the analysis concerned the current international scenario. The purpose is to separate the theoretical concept of constitutional rigidity from the ways in which it is concretely guaranteed.

Cybercrime e nuove forme di aggressione al patrimonio. Un’indagine in prospettiva europea e comparata

Negli ultimi anni si è assistito ad una rapida evoluzione degli attacchi cibernetici contro il patrimonio e, più in generale, le infrastrutture critiche. I c.d. cyber-attacchi garantiscono ai criminali informatici ed in specie ad organizzazioni criminali la possibilità di conseguire lauti guadagni a fronte di limitate probabilità di incorrere in sanzioni penali, potendo contare sulla scarsa attenzione e sull’impreparazione delle vittime e potendo celare la loro vera identità dietro l’anonima...

Turismo 'sostenibile' e itinerari turistici per la valorizzazione dei territori rurali

The research work considers the characteristics, evolution, and territorial impacts of tourism. Moreover it analyses the evolution of geographical studies on tourism, until the most recent trends. A focus is set on the sustainability of tourism, with particular regard to the most important international documents concenrning the environment, local communities and cultural heritage. Routes for tourism and culture are considered priviledge instruments to promote the sustainability of tourism. A...

Homologous recombination DNA repair gene alterations identify a subset of pancreatic cancers potentially responding to platinum based therapy

Background: The International Cancer Genome Consortium (ICGC) whole genome sequencing effort identified an average of 26 mutations per pancreatic ductal adenocarcinoma (PDAC). KRAS mutations are the hallmark, followed by TP53, SMAD4 and CDKN2A inactivation. A dominating tail of decreasingly mutated genes follows, but individual pathogenic gene alterations aggregate into ten core molecular pathways, one of which is the homologous recombination (HR) DNA repair genes pathway. Aim: Within this fr...

Establishment and characterization of two human miR-34a knockout cell lines, HeLa and HEK293T, applying the CRISPR/Cas9 system.