The combined evaluation of physiology and behaviour allows a complete and comprehensive pre-clinical assessment of central nervous system (CNS) functions. An integrated video-telemetric electroencephalography (Video-tEEG) system, which allows the simultaneous and continuous recording of EEG and video images for long periods, was developed. This research work focuses on the equipment set-up and surgical methodology for combined recording of cortical, hippocampal EEG and electromyogram (EMG) waveforms by telemetry in freely moving rats. The post-operative recovery of animals was monitored by recording of EEGs and the general activity by videos for approximately 24 hours on Day 1, 6 and 15 after surgery. The results suggest that the applied surgical technique for the implantation of telemetric transmitter allows a gradual recovery of animals within 15 days. During all the recovery period the behavioural and locomotor parameters showed that there were no changes of the light-dark circadian cycle and they return to background values within a 15-day period. Using a mechanical connection between the deep and the telemetric electrodes, the recording system is able to acquire hippocampal EEG of good quality starting from 15 days after surgical implantation. The present research work demonstrated the possible use of this surgical technique and the application of the integrated Video-tEEG system in different pre-clinical research areas and also in Safety Pharmacology, with advantages for an ethical use of animals.
An integrated system for video and telemetric EEG recording, measuring beavioural and physiological parameters, as a tool in safety pharmacology testing for assessing CNS-side effects
MOSCARDO, ELENA
2009
Abstract
The combined evaluation of physiology and behaviour allows a complete and comprehensive pre-clinical assessment of central nervous system (CNS) functions. An integrated video-telemetric electroencephalography (Video-tEEG) system, which allows the simultaneous and continuous recording of EEG and video images for long periods, was developed. This research work focuses on the equipment set-up and surgical methodology for combined recording of cortical, hippocampal EEG and electromyogram (EMG) waveforms by telemetry in freely moving rats. The post-operative recovery of animals was monitored by recording of EEGs and the general activity by videos for approximately 24 hours on Day 1, 6 and 15 after surgery. The results suggest that the applied surgical technique for the implantation of telemetric transmitter allows a gradual recovery of animals within 15 days. During all the recovery period the behavioural and locomotor parameters showed that there were no changes of the light-dark circadian cycle and they return to background values within a 15-day period. Using a mechanical connection between the deep and the telemetric electrodes, the recording system is able to acquire hippocampal EEG of good quality starting from 15 days after surgical implantation. The present research work demonstrated the possible use of this surgical technique and the application of the integrated Video-tEEG system in different pre-clinical research areas and also in Safety Pharmacology, with advantages for an ethical use of animals.File | Dimensione | Formato | |
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https://hdl.handle.net/20.500.14242/113831
URN:NBN:IT:UNIVR-113831