EUKARYOTIC ELONGATION FACTOR 2 KINASE DOWNREGULATES VESICLE RELEASE AND GABAERGIC TRANSMISSION BY TRANSLATION CONTROL OF A SUBSET OF PROTEINS

eEF2K, also known as CAMKIII, is a highly regulated, ubiquitous kinase involved in the control of protein translation. In neurons, acute changes in eEF2K activity are associated with changes in synaptic proteome and function in vitro and in vivo. However, it is unclear what kind of synaptic proteomic and functional changes result from chronic alterations in eEF2K activity. Here we show that chronic eEF2K activity leads to a downregulation of vesicle release and the GABAergic system both in vitro and in vivo. Importantly, we show that eEF2K may be a potential target for the generation of antiepileptic drugs.