Development and manufacture of a miniatured flow through-put (multiple) bioreactor system

Development and Manufacture of a miniatured flow through-put (multiple) bioreactor system PhD-Thesis, University of Rome – Tor Vergata, 2015, 163/174 Pages, 73 Figures, 14 Tables, 123 References. Bioreactor systems for cultivating cells in Life Sciences have been widely used for decades. Recently, there is a trend towards miniaturized and even microsized systems, fulfilling increasing demands strongly aiming for production and testing of novel pharmaceutical products. The aim of this PhD thesis is to develop and to manufacture such a disposable miniaturized multiple bioreactor system aiming for low cost mass production of such devices implementing artificial lymph nodes. A recursive strategy is necessary for optimizing the design and the manufacture of such artificial lymph nodes. The designs of the components and the final reactor system additionally have to be compatible to low costs manufacturing via injection moulding. Furthermore, the applied polymeric material has to be biocompatible as well as resistant to sterilisation by means of hard gamma-ray radiation. The final task includes the joining techniques in order to ensure a fluidic sealing of each single reactors as well as the whole reactor system.