The Art of Formulation od Perfluorocarbon Encapsulated Polymeric PLGA Nanoparticles

This research work is focused on the encapsulation of perfluoro-15-crown-ether (PFCE) within polymeric NPs using the PLGA polymer as a contrast agent (CA) for MRI and examines multiple facets of the formulation process. The thesis is structured into 5 chapters : Chapter 2 “Nanotechnology as a Versatile Tool for 19F-MRI Agent’s Formulation: A Glimpse into the Use of Perfluorinated and Fluorinated Compounds in Nanoparticles” : This chapter offers an in-depth literature review on cutting-edge carrier systems for encapsulating PFCs. It provides insights into the utilization of perfluorinated and fluorinated compounds within NP frameworks. Chapter 3 “The Art of Preparations of PFCE Encapsulated PLGA Nanoparticles : Formulation and optimisation using High Speed Homogenization and Ultrasonication” The focus of this chapter is the formulation and optimization of PLGA NPs containing perfluoro-15-crown-ether (PFCE). It examines the impact of varying molecular weights of PLGA and the role of surfactants in the formulation process, utilizing techniques such as homogeniser and ultrasonic sonication for emulsion formation. Chapter 4 “Central Composite Design of Experiment Based Optimization of Key Formulation Parameters for PFCE- Encapsulated PLGA Nanoparticles”. A statistical approach is adopted to explore the parameters critical to the nanoformulation of PLGA-PFCE. This chapter delves into the methodical aspects that underpin the successful preparation of these NPs. Chapter 5 “Strategic Enhancement of PLGA Nanoparticles: PEG and Fluorine Additions for PFCE Encapsulation” – These chapters document the exploration of PLGA modification with polyethylene glycol (PEG) to increase the circulation time of the NPs and fluorine to increase the PFCE loading, employing advanced cerium catalysts. The modifications aim to enhance the preparation process of PFCE-encapsulated PLGA NPs. Finally, Chapter 6 “Conclusion and future perspectives” This section provides a comprehensive overview and analytical examination of the results derived from this thesis. It also contemplates potential enhancements and the broader clinical implementation of the formulations.