Application of intronic markers to sustainable management of natural fish resources

The identification and traceability of fish resources, including species, populations, and stocks, are essential for sustainable fisheries management and biodiversity conservation. This doctoral research developed and optimized a novel genetic methodology utilizing Multi-locus Intron Polymorphisms (MIPs). The study explores their application as robust molecular markers for species identification, population structure analysis, and the detection of interspecific hybridization. Methodological advancements include the development of locus-specific primers targeting conserved exon-intron regions and the creation of an automated bioinformatics pipeline for efficient data analysis. MIPs were applied across various case studies, demonstrating their versatility in distinguishing closely related species and hybrids in diverse taxa. Additionally, the research addressed the challenges posed by polyploid genomes, particularly in sturgeons, by assembling and annotating a high-quality reference genome for the critically endangered Adriatic sturgeon (Acipenser naccarii). In collaboration with Eurofishmarket, the findings were integrated into practical frameworks to enhance resource management and promote sustainable fisheries practices. This project underscores the potential of MIPs as innovative tools in molecular genetics, bridging the gap between research and industry while supporting conservation efforts and the sustainable use of aquatic resources.