Proteomic tools in clinical tissues: unlocking the pathology archives

This study demonstrated that it is possible to harmonize protein analysis in archive tissues in different European laboratories by using the same protocols for tissue processing and protein extraction. ? The optimization of an appropriate protocol for molecular analysis of tissues fixed with new formalin-free fixative FineFix. The possibility of using proteomic approaches also on tissues fixed with reagents alternative to formalin has great potential for future pathology, when the use of formalin will be banned due to its carcinogenetic effects. Furthermore, FineFix has proven a suitable formalin substitute in clinical practice, because it preserves both specimens' morphology and immunoreactivity. ? The validation of a new antibody against the E7 oncoviral protein for the detection f HPV in cervical squamous lesions. This leads us to conclude that E7 might be a suitable specific marker for the diagnosis of cervical lesions and could be used also for others HPV-linked diseases. The analysis of a battery of E7 monoclonal antibodies has highlighted their great usefulness in differential diagnosis. ? An in-depth knowledge of the function of RECQ1 in glioblastoma. RECQ1 expression has been studied in glioblastoma cell lines for the first time ever. This research has demonstrated its important role in glioblastoma growth and proliferation, and in maintaining genome stability. ? The recommendation of RECQ1 helicase as therapy target in glioblastoma, based on the evidence that the enzyme expression in