Development of electrochemical sensors by nanofabrication techniques for biological and medical diagnostics.

In this work the fabrication and characterization of boron-doped diamond (BDD) nanoelectrode arrays are discussed. NEAs were obtained by creating an array of nanoholes by electron beam lithography (EBL) and Nanoimprint Lithography in a thin film of polycarbonate deposited on top of the macroelectrode. This approach leads to the formation of recessed nanoelectrodes. NEAs have been characterized with cyclic voltammetry and have provided voltammetric signals controlled by pure radial diffusion. Short ss-DNA have been immobilized on polycarbonate surface and hybridization experiments were carried out. Fluorescent and enzymatic labels were used in order to detect immobilization and hybridization of DNA. ECL preliminar experiment were also carried out on our NEAs.