RESEARCH AND DEVELOPMENT THROUGH AUTOMATED LABORATORY PROCEDURES

UNILAMELLAR VESICLES, SIGNIFICANTLY OUTPERFORMING TRADITIONAL INDIUM TIN OXIDE (ITO) METHODS IN TERMS OF COST, DURABILITY, AND EFFICIENCY. THE PLATFORM PROVED ROBUST FOR HANDLING COMPLEX TERNARY MIXTURES (POPC:SM:CHOL) AND WAS SUCCESSFULLY APPLIED TO INVESTIGATE MEMBRANE RIGIDITY VIA GENERALIZED POLARIZATION (GP) AND TO VALIDATE THE PASSIVE TRANSLOCATION OF CELL-PENETRATING PEPTIDES (CPPS). II FINALLY, TO BRIDGE THE GAP BETWEEN RAW DATA AND BIOLOGICAL INSIGHT, A COMPREHENSIVE COMPUTATIONAL FRAMEWORK WAS DEVELOPED. THIS SUITE INCLUDES UTILITIES FOR CROSS-PLATFORM SPECTRAL DATA STANDARDIZATION (DX2CSV) AND AN ADVANCED DECONVOLUTION TOOL (EXTRAPOLATOR). THE LATTER SIGNIFICANTLY ENHANCES THE SENSITIVITY OF MEMBRANE FLUIDITY MEASUREMENTS BY RESOLVING OVERLAPPING SPECTRAL COMPONENTS, OFFERING A MORE ROBUST METRIC THAN TRADITIONAL INTENSITY-BASED METHODS. COLLECTIVELY, THIS WORK DEMONSTRATES THAT HIGH-QUALITY BIOPHYSICAL DATA CAN BE OBTAINED USING ACCESSIBLE, CUSTOM-BUILT INSTRUMENTATION. THE DEVELOPED PLATFORMS OFFER A SCALABLE AND FLEXIBLE WORKFLOW FOR HIGH-THROUGHPUT MEMBRANE RESEARCH, PAVING THE WAY FOR MORE EFFICIENT DRUG SCREENING AND FORMULATION DEVELOPMENT.