Development of image-based high content screening assays for testing monoclonal antibodies against Neisseria gonorrhoeae infecting human cells

Monoclonal antibodies (mAbs) represent a promising class of therapeutics against bacterial pathogens, including those which are developing antimicrobial resistance (AMR). Functional, high-throughput assays, which investigate the ability of mAbs to kill bacteria or protect the host, are currently lacking. The aim of this PhD thesis was to establish high-content and high-throughput methods to screen and characterize functional mAbs against Neisseria gonorrhoeae.