Oltre la lisi cellulare batterica: l’impatto delle concentrazioni sub-inibitorie di antibiotici sulla formazione del biofilm

La formazione di biofilm sui dispositivi medici rappresenta una rilevante sfida clinica, poiché costituisce un elemento chiave nello sviluppo e nella persistenza delle infezioni. In particolare, quando tali dispositivi sono esposti al flusso di fluidi, si osserva frequentemente la comparsa di biofilm filamentosi, o streamer. In queste strutture sospese, il DNA extracellulare (eDNA) funge da componente portante essenziale ed è particolarmente rilevante nei dispositivi a permanenza, come i cate...

Patient-specific Computational Modelling of TAVI: Engineering Advances Toward Clinical Application

Transcatheter Aortic Valve Implantation (TAVI) has emerged as a pivotal therapeutic intervention for patients with severe aortic stenosis who are considered unsuitable for conventional open-heart surgery. Despite its clinical efficacy, pre-procedural planning remains challenging due to the complexity of patient-specific anatomies and the intricate biomechanics associated with device deployment, particularly in anatomically complex cases. Computational modelling has increasingly been recognize...

Intratumoral E.coli T30 in breast cancer: impact on CHI3L1 and PD-L1 expression and its immunomodulatory effects

Breast cancer is a multifaceted disease influenced by a variety of factors, including the tumor microenvironment and the host microbiota. Emerging evidence has highlighted the presence of intratumoral bacteria in different cancer types, with potential implications for tumor progression and immune modulation. In this study, we characterized an intratumoral Escherichia coli strain, E. coli T30, previously isolated from the 4T1 murine breast cancer model in our laboratory. Whole-genome sequencin...

IgA and Macrophages Modulation in the Choroid Plexus: Bridging Adaptive and Innate Immunity in the Gut-Brain Axis

Tackling bacterial infections of the bone: PTX3 as new diagnostic tool and therapeutic target

Osteomyelitis (OM) is a severe musculoskeletal infection mainly caused by the opportunistic bacterium S. aureus (SA) that occurs after fracture, surgery, or secondary to vascular spread of the pathogen to skeletal sites. Several factors, including presence of foreign bodies (implants) and dysmetabolic diseases (diabetes), make the bone susceptible to infections. OM manifests with extensive tissue remodeling and inflammation. Host immune reactions and SA survival strategies coordinately affect...

Combined immunotherapy with TLR ligands in pancreatic cancer elicits strong anti-tumor response and boosts interferon-mediated killing of tumor cells

The incidence and the lethality of pancreatic ductal adenocarcinoma (PDAC) is alarmingly increasing in the last decades worldwide and novel therapies are urgently needed to face this malignancy. In this project, we investigated the therapeutic potential of poly(I:C) (pIC) and resiquimod (R848), two Toll-like receptor agonists, in different preclinical models of PDAC. We found that, when used in combination, pIC+R848 elicit a potent antitumoral response in all the in vivo models investigated, ...

Total marrow and lymph nodes irradiation in conditioning regimen for allogeneic hematopoietic stem cell transplantation Artificial Intelligence algorithms to automate the Total Marrow and Lymph-node Irradiation by VMAT optimization using WB-CT/MRI and synthetic WB-CT - The AutoMI project

Purpose/Objective The AuToMI project focuses on improving the TMLI clinical workflow and automating radiotherapy procedures through the application of Artificial Intelligence (AI). The primary goal is to streamline and enhance the efficiency of the radiotherapy treatment process by automating two critical steps: contouring of the target volumes and treatment plan optimization. In parallel, the project aims to integrate whole-body magnetic resonance imaging (WB-MRI) to improve the accuracy of ...

Total marrow and lymph nodes irradiation in conditioning regimen for allogeneic hematopoietic stem cell transplantation Artificial Intelligence algorithms to automate the Total Marrow and Lymph-node Irradiation by VMAT optimization using WB-CT/MRI and synthetic WB-CT - The AutoMI project

Purpose/Objective The AuToMI project focuses on improving the TMLI clinical workflow and automating radiotherapy procedures through the application of Artificial Intelligence (AI). The primary goal is to streamline and enhance the efficiency of the radiotherapy treatment process by automating two critical steps: contouring of the target volumes and treatment plan optimization. In parallel, the project aims to integrate whole-body magnetic resonance imaging (WB-MRI) to improve the accuracy of ...

Total marrow and lymph nodes irradiation in conditioning regimen for allogeneic hematopoietic stem cell transplantation Artificial Intelligence algorithms to automate the Total Marrow and Lymph-node Irradiation by VMAT optimization using WB-CT/MRI and synthetic WB-CT - The AutoMI project

Purpose/Objective The AuToMI project focuses on improving the TMLI clinical workflow and automating radiotherapy procedures through the application of Artificial Intelligence (AI). The primary goal is to streamline and enhance the efficiency of the radiotherapy treatment process by automating two critical steps: contouring of the target volumes and treatment plan optimization. In parallel, the project aims to integrate whole-body magnetic resonance imaging (WB-MRI) to improve the accuracy of ...